|SI No||Time (min)||E Gene||S Gene|
|CT Value||Delta CT||% reduction in viral load||CT Value||Delta CT||% reduction in viral load|
|2||15||29||3||> 90%||28||3||> 90%|
|4||60||ND||-||End point||-||End point||End point|
|5||120||ND||-||End point||-||End point||End point|
The virus has been engineered to express GFP gene for monitoring the virus on infection of host cells.
Results show that MDCK cells infected with virus pretreated on tekFABRIK TM show no GFP fluorescence virus spotted on control cloth were live and infected MDCK cells as exhibited GFP fluorescence, This confirmed that chemically modified TekFABRIK has viricidal properties against H1N1.
|SI No||Sample Name||Batch Number||Product Code||Result|
|1||tekFABRIK TM||CCMP-0420-005||IS-210||GFP signal same as the untreated virus control|
|1||tekFABRIK TM||CCMP-0420-005||IS-212||No GFP signal detected|
|1||tekFABRIK TM||CCMP-0420-005||IS-216||No GFP signal detected|
tekFABRIK TM was tested for its viricidal activity against RSV in a US academic laboratory that has studied RSV for many years and has developed a sensitive luciferase-based assay to monitor RSV infection.
The genetically engineered RSV (rA2-Rluc) was incubated with tekFABRIK TM according to a modified ISO 18184 procedure. At the indicated times, the virus was recovered from the cloth and virus infectivity measured by incubation with A549 cells, a human lung epithelial line ( (ATCC, CCL-185). tekFABRIK TM had high viricidal activity against RSV with 97% reduction in viral infectivity after 5 min as determined in three independent experiments.Method Reference: Development of a simple, rapid, sensitive, high-throughput luciferase reporter based microneutralization assay for respiratory syncytial virus. Fuentes S, Crim RL, Beeler J, Teng MN, Golding H, Khurana S. Vaccine 2013; 31:3987-94.
Phages are non- enveloped virus that infect E.coli and are routinely used a model to test a for viricidal activity. Also, bacteriophages can be studied in a standard research lab with biosafety level 1 Bacteriophage was spotted on tekFABRIK TM / control fabric for different time periods and then plated by Agar overlay assay. After 24 h, the plates were observed for plaques. Alternatively, the E.coli infected with phages is directly plated on LB agar to determine the viricidal activity.
|Bacteria only||N/A||Virus conc||3.9x105|
|Bacteria + phage||5 min||1/10 dilution of neat||No colonies|
|Bacteria + pohase pretreated with cotton||1/10 dilution of neat||No colonies|
|Bacteria + phage pretreated with IS212 cotton||1/10 dilution of neat||4.1x105|
100% killing of phage
TekFABRIK has been extensively studied and shown to have broad – spectrum anti- bacterial properties encompassing both gram positive and gram negative bacteria. For example, two strains commonly associated with hospital acquired infections, Staphylococcus aureus (gram positive) and Klebsiella pneumoniae (gram negative) were tested and the results are shown below. An inoculum of 1-2 x105 in PBS was spotted on TekFABRIK of control cloth and incubated for the indicated times. The bacterial cells were harvested from both the TekFABRIK and control cloth and plated on Tryptic soy agar and incubated overnight at 37°C. The number of colony forming units (cfu) was measured and converted to % kill as a function of time using the formula:
(cfu/mL (control cloth) – cfu /mL(tekFABRIK)/Total cells spotted) x 100 = % Kill
|Staphylococcus aureus||Klebsiella pneumoniae|
|Time (in min)||Control cloth||tekFABRIK TM||% killing||Control cloth||tekFABRIK TM||% killing|
|10||1.54x105||No colonies3||100||1.34x105||No colonies3||100|
|10||1.38x105||No colonies3||100||1.21x105||No colonies3||100|